AIM: to practice using some sterile techniques, to examine a range of micro-organisms found in descent macroscopically and to examine a range of micro- organisms found in intellectual nourishment and water supply macroscopically APPARATUS:         Incubator         unfertilized nutritive agar plates 1.         3 water ensamples 2.         1 air sample 3.         3 food samples         etna burner         Sterile pipettes         aver loops         Sticky tape         Marking tape         Water samples(various)         yogurt         Inoculating needle/ probe         sick from fruit, vegetables or cultivated cabbage         Methylated strong drink RISK ASSESMENT: 1.         Glassw be - tolerate cause cuts if handled incorrectly 2.         Bunsen burner - can cause burn down if not on caoutchouc kindle 3.         Methylated hard drink - toxic if ingested, highly combustible. Skin irritant 4.         Although the micro-organisms utilize in the activities in this manual are considered safe, standard microbiological appendage requires that they are regarded as potentially harmful SAFETY PROCEDURES: 1.         Handle with care, do not feed alone glassware near the surround of the bench 2.         take care, do not leave flame unattended keep flammable liquid i.e. Methylated spirit up away 3.         Keep Methylated spirits away from flames. break in gloves when handling it 4.         Wear gloves, lab coat and safety glasses. each used equipment should be placed in disinfectant. Dispose of cultures after use, in an autoclave. Wash hands forrader leaving lab. never open agar plate after setting up METHOD: 1.         Sterilise the workbench area by wipe ! up alcohol on to it 2.         collect 8 sterile wholesome agar plates 3.         A sample of youthful milk was obtained 4.         leave one plate aside, certain and taped as the oblige 5.         use a sterile inoculating loop, a nice sample of the fresh milk was streaked onto the plates. This plate was thus sealed with sticky tape and labeled as fresh milk 6.         travel 3 and 4 were then repeated with tap water, distilled water, seek tank water, fresh milk, old milk, If you ask to get a full essay, order it on our website: OrderEssay.net
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